Sofosbuvir Impurity 35 (Crude) is a synthetic byproduct arising from incomplete phosphorylation during the final stages of Sofosbuvir production. It retains the 2’-C-methylribofuranose backbone but lacks the terminal phosphate ester, featuring a free hydroxyl group at the 5’-position. This structural deficiency in the phosphorothioate linkage renders it a critical HPLC reference standard for monitoring process consistency.
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